CYP1A1与PPAR-γ在猪肺炎支原体感染炎性反应调控中的作用关系

doi:10.11843/j.issn.0366-6964.2016.03.021

Abstract

Abstract:

In order to study the effect of CYP1A1 expression change in Mycoplasma hyopneuminiae infection and to investigate the association betweenCYP1A1and peroxisome proliferator-activated receptors associated-γ (PPAR-γ)，we obtained the full-length coding sequence of CYP1A1 by means of cloning and eukaryotic expression vector construction method，established PAMs cell lines that stably expressed CYP1A1 by method of cell-transfection and resistance screening techniques，constructed cell experimental model infection with Mycoplasma hyopneuminiae and analyzed the relationship of the expression level of PPAR-γ and CYP1A1.The results show that the expression level of PPAR-γ is positively correlated with CYP1A1 and is involved in the regulation of inflammation，which suggest that CYP1A1 may play a role in suppressing inflammation by regulating PPAR-γ.

CLC Number:

S852.62

XU Jie,ZHAO Wei-min,REN Shou-wen，ZHAO Fang,ZHOU Yan-hong,LI Hui-xia，FANG Xiao-min. Interaction Effect of CYP1A1 and PPAR-γ in the Regulation of Inflammation of Mycoplasma Pneumonia in Swine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 574-580.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.03.021

https://www.xmsyxb.com/EN/Y2016/V47/I3/574

References

［1］胡之璧，贺丽虹，赵淑娟.细胞色素P450研究进展［J］.江苏中医药，2006，27(10)：81-84.
HU Z B，HE L H，ZHAO S J.Research progress of cytochrome P450［J］.Jiangsu Journal of Traditional Chinese Medicine，2006，27(10)：81-84.(in Chinese)
［2］徐彬，杨忠英，樊洪忠，等.细胞色素P450与药物代谢性相互作用［J］.山西医药杂志，2015，44(12)：1376-1380.
XU B，YANG Z Y，FAN H Z，et al.Interaction effects of Cytochrome P450 and drug metabolism［J］.Shanxi Medical Journal，2015，44(12)：1376-1380.(in Chinese)
［3］MORGAN E T.Impact of infectious and inflammatory disease on cytochrome P450-mediated drug metabolism and pharmacokinetics［J］.Clin Pharmacol Ther，2009，85(4)：434-438.
［4］RENTON K W.Alteration of drug biotransformation and elimination during infection and inflammation［J］.Pharmacol Ther，2001，92(2-3)：147-163.
［5］PASZTI-GERE E，MATIS G，FARKAS O，et al.The effects of intestinal LPS exposure on inflammatory responses in a porcine enterohepatic co-culture system［J］. Inflammation，2014，37(1)：247-260.
［6］ISSEMANN I，GREEN S.Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators［J］. Nature，1990，347(6294)：645-650.
［7］ADAMS M，REGINATO M J，SHAO D，et al.Transcriptional activation by peroxisome proliferator-activated receptor γ is inhibited by phosphorylation at a consensus mitogen-activated protein kinase site［J］.J Biol Chem，1997，272(8)：5128-5132.
［8］ZHU Y，KAN L，QI C，et al.Isolation and characterization of peroxisome proliferator-activated receptor (PPAR) interacting protein (PRIP) as a coactivator for PPAR［J］.J Biol Chem，2000，275(18)：13510-13516.
［9］JIANG C，TING A T，SEED B.PPAR-γ agonists inhibit production of monocyte inflammatory cytokines［J］. Nature，1998，391(6662)：82-86.
［10］WANG N，VERNA L，CHEN N G，et al.Constitutive activation of peroxisome proliferator-activated receptor-γ suppresses pro-inflammatory adhesion molecules in human vascular endothelial cells［J］.J Biol Chem，2002，277(37)：34176-34181.
［11］王延军.细胞色素P450表达的诱导机制及其筛选方法的研究进展［J］.内蒙古医学杂志，2008，40(3)：332-334.
WANG Y J.Research progress of induction mechanism and screening method of the expression of cytochrome P450［J］.Inner Mongolia Medical Journal，2008，40(3)：332-334.(in Chinese)
［12］宋汉香，王淑秋，吕少春，等.芳香化酶基因敲除小鼠肝组织PPARα表达及与肝脂肪变性的关系［J］.中国老年学杂志，2012，32(13)：2766-2769.
SONG H X，WANG S Q，LV S Q，et al.Relationship between the expression of PPARα in liver tissue which is Aromatase knockout and liver steatosis［J］.Chinese Journal of Gerontology，2012，32(13)：2766-2769.(in Chinese)
［13］刘旺根.雌激素受体-α 36过表达对人乳腺癌细胞MCF-7生物学行为的影响［D］.郑州：郑州大学，2012.
LIU W G.The Effect of estrogen receptor alpha 36 overexpression on biological behaviors of human breast cancer cell MCF-7［D］.Zhengzhou：Zhengzhou University，2012.(in Chinese)
［14］BLANQUART C，BARBIER O，FRUCHART J C，et al.Peroxisome proliferator-activated receptors：regulation of transcriptional activities and roles in inflammation［J］.J Steroid Biochem Mol Biol，2003，85(2-5)：267-273.
［15］LIANG Y，ZHOU Y，SHEN P.NF-kappaB and its regulation on the immune system［J］.Cell Mol Immunol，2004，1(5)：343-350.
［16］STRAUS D S，PASCUAL G，LI M，et al.15-Deoxy-Δ12，14-prostaglandin J2 inhibits multiple steps in the NF-κB signaling pathway［J］.Proc Natl Acad Sci U S A，2000，97(9)：4844-4849.
［17］RASMUSSEN M K，IVERSEN L，JOHANSEN C，et al.IL-8 and p53 are inversely regulated through JNK，p38 and NF-κB p65 in HepG2 cells during an inflammatory response［J］.Inflamm Res，2008，57(7)：329-339.
［18］曾锦章.细胞信号传导与转录因子AP-1活性的调节［J］.国外医学遗传学分册，1999，22(4)：191-194.
ZENG J Z.Regulation of the activity of cell signaling transcription factor-AP-1［J］.Section of Genetics Foreign Medical Sciences，1999，22(4)：191-194.(in Chinese)
［19］CHEN S，OPERAÑA T，BONZO J，et al.ERK kinase inhibition stabilizes the aryl hydrocarbon receptor：implications for transcriptional activation and protein degradation［J］. J Biol Chem，2005，280(6)：4350-4359.
［20］SHIBAZAKI M，TAKEUCHI T，AHMED S，et al.Suppression by p38 MAP kinase inhibitors (pyridinyl imidazole compounds) of Ah receptor target gene activation by 2，3，7，8-tetrachlorodibenzo-p-dioxin and the possible mechanism［J］.J Biol Chem，2004，279(5)：3869-3876.

[1]	XIE Qingyun, XING Huixuan, YU Yanfei, YUAN Ting, XIONG Qiyan, XIONG Fuqiang, FENG Zhixin. Prokaryotic Expression, Polyclonal Antibody Preparation and Activity Identification of Helicase RuvA from Mycoplasma hyopneumoniae [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 271-281.
[2]	CHEN Qiwei, QUAN Heng, CHEN Shengli, LIU Donghui, YU Yongfeng, LI Caiyu, GONG Xiaowei, CHU Yuefeng. Functional Analysis of Biotin Uptake by the Energy-coupled Factor Transporter S Component MbBioY of Mycoplasma bovis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 282-289.
[3]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[4]	ZHONG Lemiao, LIU Binghui, WU Chunlin, WU Yijian. Preparation and Evaluation of Subunit Vaccine based on Adhesin Protein of Mycoplasma gallisepticum [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5171-5183.
[5]	WU Qi, ZHANG Yujuan, LIU Tong, XIN Jiuqing, XU Qingyuan. Research Progress of Mycoplasma bovis Adhesins and Adhesin-binding Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3206-3216.
[6]	JING Tingting, YIN Hao, HUANG Rong, LAN Shimei, LI Zhangcheng, YOU Liuchao, HAO Huafang, FU Lei, CHU Yuefeng. Prokaryotic Expression and Biological Function Analysis of Mycoplasma bovis 0580 Gene C-terminal Truncated [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2991-3001.
[7]	CHEN Yang, MENG Linchun, GUO Mengjiao, ZHANG Chengcheng, BO Zongyi, CHU Dianfeng, CAO Yongzhong, WU Yantao, ZHANG Xiaorong. Establishment and Preliminary Application of Indirect ELISA Method and HI Test for Detection of Mycoplasma Gallisepticum Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2062-2072.
[8]	WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.
[9]	XU Lifeng, JIA Chenyu, CHEN Fuzai, FANG Mengyuan, LIU Xiaodan, CHEN Jilong, LI Xunliang. Isolation, Identification and Whole Genome Analysis of a Mycoplasma synoviae Strain from Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2663-2676.
[10]	ZENG Jinhong, XU Qingyuan, SHA Weiping, WU Shuang, LI Yuan, XIN Jiuqing, WANG Xiumei. Development and Application of Monoclonal Antibodies against Mycoplasma mycoides subsp.Mycoides [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2290-2299.
[11]	YIN Dejing, WU Yan, YUE Jun, YANG Peng, CHEN Jing, WANG Hui, ZHANG Shuangxiang, WANG Kaigong, CHENG Zhentao. Construction of Eukaryotic Expression Vector of N-terminal Gene of LppA Protein of Mycoplasma Mycoides subsp.Capri and Analysis of Its Immune Efficacy in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 847-856.
[12]	LIU Lijia, WANG Yu, ZHANG Huanrong, WANG Jiabo. Whole Gene Analysis of 7 Mycoplasma synoviae Strains from Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 505-519.
[13]	YUE Yahui, XING Xiaoyong, WU Xiaochun, WEN Fengqin, ZHANG Hongyan, LONG Cuiqin, ZHANG Li, MA Haiyun, BAO Shijun. Expression of Elongation Factor Thermo Unstable of Mycoplasma synoviae WVU1853 Strain and Analysis of Its Adherence Characterization [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 520-528.
[14]	WANG Jia, HUA Lizhong, LIU Beibei, ZHANG Lei, YUAN Ting, GAN Yuan, WEI Yanna, SHAO Guoqing, FENG Zhixin, XIONG Qiyan. Isolation, Identification and Pathogenicity of a Mycoplasma hyorhinis Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3185-3193.
[15]	CHEN Shengli, HAO Huafang, JI Wenheng, CHU Yuefeng. Research Advances in Immunogenicity of Related Proteins of Animal Mycoplasmas [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(5): 1230-1237.

Interaction Effect of CYP1A1 and PPAR-γ in the Regulation of Inflammation of Mycoplasma Pneumonia in Swine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments